Figure 8: Perturbation of the Foxa2-vCP population results in aberrant cardiac morphology.
From: Foxa2 identifies a cardiac progenitor population with ventricular differentiation potential
(a,b) WT (left) and Foxa2Cre:Isl1lox/lox (right) embryos were imaged for morphological differences at E9.0 (a) and E10.0 (b). (c) IF analysis of cryosectioned E9.5 WT and Foxa2Cre:Isl1lox/lox embryos with antibodies against cTnT and Mlc2v. (d) WMIF and confocal z-projection of E9.5 WT and Foxa2Cre:Isl1lox/lox embryos with antibodies against Nkx2–5 and cTnT to label the growing heart tube. (e,f) WMIF analysis of E8.5 Foxa2Cre:YFP embryos injected at E7.5 with either dimethylsulfoxide (DMSO; vehicle control, top rows) or RA (65 mg kg−1, bottom rows) and labelled with antibodies against YFP, Nkx2–5 and either Hcn4 (e) or Isl1 (f). Brackets indicate expansion of heart field region after RA treatment. Images shown are representative (n=3 embryos for each stain). PV, primitive ventricle; CC, cardiac crescent; SHF, second heart field. Scale bars, 75 μm (c) or 100 μm (d,e).
