Figure 2: Effect of SIRT6 depletion on expression of a telomeric luciferase transgene.
From: SIRT6 is required for maintenance of telomere position effect in human cells
(a) Relative luciferase activity in cells harbouring a telomeric (left) or internal (right) luciferase transgene, transfected with control siRNA or siRNA targeting SIRT6. Cells were collected for luciferase assays ∼72 h after siRNA transfection. (b) Relative luciferase activity in a second independent set of telomeric and internal luciferase cell lines, transfected with SIRT6-directed siRNA and collected as in (a). (c) Relative luciferase activity in cells transfected with control siRNA or two independent SIRT6 siRNAs, collected as in (a). (d) Relative luciferase activity in cells transfected with control siRNA or siRNA targeting SIRT1 and collected as in (a,c). For a–d, luciferase activity is normalized to control siRNA-transfected cells; results are from three independent siRNA transfections; error bars represent ±s.d. (e) Telomeric luciferase cells expressing ectopic hTERT were transfected with the indicated siRNAs, and luciferase assays were performed at the indicated time-points after transfection. Luciferase activity is normalized to control siRNA-transfected cells at each time-point; results are from three independent siRNA transfections; error bars represent ±s.d.
