Figure 5: Effect of SIRT6 depletion on expression of ISG15, an endogenous telomere-proximal gene.
From: SIRT6 is required for maintenance of telomere position effect in human cells
(a) ISG15 mRNA levels in vector- and hTERT-transduced HeLa cells were determined by reverse transcription and quantitative real-time PCR and normalized first to β-actin levels and then to vector-transduced cells. (b) ISG15 mRNA levels in HeLa cells transfected with control siRNA or si SIRT6 no. 1 were determined as in (a) and normalized first to β-actin and then to control siRNA-transfected cells. Light grey bars, si ctrl; dark grey bars, si SIRT6. Data show the average of three replicates ±s.e.m., and are representative of three independent experiments.
