Figure 1: Identification of exosome miRNA profile that represents primary colon cancer and metastatic colon cancer in the liver.
From: MVP-mediated exosomal sorting of miR-193a promotes colon cancer progression

(a) Schematic diagram for isolation of extracellular vesicles (EVs) from colon cancer CT26 cell line with multimodal imaging report. CT26 cells stably transduced with a lentiviral vector expressing membrane-bound Gaussia luciferase (GlucB) and biotin ligase (BirA). (b) Venn diagram summarizing unique and shared exosomal miRNAs detected in the tissues of naïve colon, primary colon cancer and metastatic mouse colon cancer in the liver using miRNA microarray data (n=5 mice per group). (c) Microarray data visualization by scatter plot comparing exosomal miRNAs detected in primary colon cancer (x axis) and metastatic colon cancer in the liver at day 3 (y axis) after a CT26 cell intrasplenic injection. (d) Heat map depicting changes in miRNAs with a statistically significant (P<0.05) change in the exosomal miRNAs from normal mouse colon, primary colon cancer tissue and metastatic colon cancer in the liver at days 3, 7 and 14 after injection of CT26 cells (n=3 mice per group). All tumour-derived exosomes were isolated with streptavidin magnetic beads. Microarray analysis results (e) and qPCR verification (f) of selected exosomal miRNAs from the source as described in d. (g) qPCR analysis of the plasma- (left panel) or faeces- (right panel) derived exosomes from the source are depicted in d. *P<0.05 versus naïve colon; #P<0.05 versus primary colon cancer (two-tailed t-test). Data are representative of three independent experiments (error bars, s.e.m.).