Figure 4: Relocalization of NIa-Pro is required to increase aphid fecundity.
From: A viral protease relocalizes in the presence of the vector to promote vector performance
Images show confocal projection of N. benthamiana leaves agroinfiltrated with (a) free GFP, (b) GFP:NIa-Pro, (c) GFP:NIa-Pro:NLS (NLS, nuclear localization signal) and (d) GFP:NIa-Pro:NES (NES, nuclear export signal) with or without aphids. (e) Callose deposition in agroinfiltrated N. benthamiana leaves with free GFP, GFP:NIa-Pro, GFP:NIa-Pro:NLS and GFP:NIa-Pro:NES with or without aphids infestation. The NLS mutant does not reduce aphid-induced callose accumulation. (f) Number of progeny produced by aphids on N. benthamiana agroinfiltrated leaves with free GFP, GFP:NIa-Pro, GFP:NIa-Pro:NLS and GFP:NIa-Pro:NES. (mean±s.e. of N=10 for e and N=8–12 for f; letters represent significant differences, ANOVA and Tukey’s honest significant difference post hoc, P<0.05). Results from one of three independent experiments are displayed. Scale bars, 20 μm.
