Figure 7: NIa-Pro relocalization and impact on aphid fecundity is host plant dependent.
From: A viral protease relocalizes in the presence of the vector to promote vector performance
(a) Images show confocal projections and a single section of transgenic A. thaliana leaves expressing free GFP or GFP:NIa-Pro with or without aphids present. (b) Number of progeny produced by aphids on healthy N. tabacum or on N. tabacum plants inoculated or agroinfiltrated with free GFP, TuMV, PVY, GFP:NIa-ProTuMV or GFP:NIa-ProPVY. (c) The percentage of cells with GFP in the vacuole for N. tabacum plants expressing free GFP, GFP:NIa-ProTuMV and GFP:NIa-ProPVY with or without aphids. (d) Images show confocal projections from c. GFP localizes to the nucleus but does not localize to the vacuole during aphid presence in N. tabacum plants. (e) The image represents a western blot made with leaf extracts from N. benthamiana or N. tabacum leaves agroinfiltrated with free GFP, GFP:NIa-ProTuMV and GFP:NIa-ProPVY, and incubated with an GFP antibody. We can see that the expression of free GFP, GFP:NIa-ProTuMV and GFP:NIa-ProPVY is similar in the different hosts. Mean±s.e. of N=6 for a,c,d and N=8–12 for b; letters represent significant differences, ANOVA and Tukey’s honest significant difference post hoc, P<0.05. Results from one out of two independent experiments are displayed in a and from three independent experiments in c. Dashed arrow indicates the vacuole and white arrows indicate the nucleus. Scale bars, 20 μm.
