Figure 8: Aif1-dependent activation of β1 integrin.

(a) Relative gene expression of β1 integrin in FAE from wild-type and Aif1^−/− mice. Data are means±s.e.m. of one experiment representative of two independent experiments (n=4 per genotype). P value was determined with Student’s t-test. (b,c) Whole-mount staining of PPs prepared from Aif1^+/+ and Aif1^−/− mice with anti-total β1 integrin antibody (b, red) or anti-activated-β1 integrin antibody (c, red), together with anti-GP2 antibody (b,c, green). Each yellow box region in c (left panels) was magnified to produce the image in c (right panels). Dotted lines indicate the periphery of the FAE region. Data are representative of three independent experiments.