Figure 1: MICAL1 localizes in late intercellular bridges at the abscission site.
From: Oxidation of F-actin controls the terminal steps of cytokinesis
(a,b) HeLa transfected with GFP-MICAL1 (green) were stained with β–tubulin (blue) and CHMP4B (red). Scale bar, 10 μm. (c) Same as in a for a CRISPR/Cas9-edited HeLa cell line expressing GFP-MICAL1endogenous. Scale bar, 10 μm. (d) Snapshots of a time-lapse fluorescent and phase-contrast microscopy movie of the GFP-MICAL1endogenous genome-edited cell line. Scale bars, 10 μm. In all figures, red arrow: midbody and orange arrow: future abscission site.
