Figure 1: RNA-seq reveals diverse classes of age-dependent rhythmicity changes. | Nature Communications

Figure 1: RNA-seq reveals diverse classes of age-dependent rhythmicity changes.

From: Circadian deep sequencing reveals stress-response genes that adopt robust rhythmic expression during aging

Figure 1

(a) Heat map representing loss or gain of gene expression rhythmicity with age. Each column represents a single-time-point for a single-replicate sampled at 4 h intervals starting at Zeitgeber Time (ZT) 0. Hue represents the Z-score (FPKM minus mean over s.d.) of each gene (row) at each time point (column) for a given biological replicate. Genes in the top half are rhythmic in young and arrhythmic in old; those in the bottom are rhythmic in old and arrhythmic in young (Methods). (b–d) RNA-seq gene expression profiles; each period of ZT 0–20 represents a distinct biological replicate. (b) Coordinated, reduced amplitude in expression of circadian genes at the Cks30A gene locus. (c) Examples of genes rhythmic only in young (CG9507 and CG11425); rhythmic in both young and old, with age-dependent increases in amplitude (RpL32 and Hr38). (d,e) ImpL3 adopts de novo rhythmic expression in old flies according to RNA-seq. Browser tracks in e show RNA-seq read pileups at each time point; reads represent merged RNA-seq data from replicate cohorts of old flies with the same y-axis range for all time points.

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