Figure 3: Identification of late-life cyclers (LLCs).
(a) Differential periodicity Z-score is plotted against differential robustness Z-score (Methods); top LLCs from Supplementary Table 3 are labelled. Data points are coloured by differential rhythmicity score (SDR). (b) Superimposed min-max normalized RNA-seq profiles of known stress-responsive LLCs with peak expression >10 FPKM. Broken lines=day 5 data; solid lines=day 55 data. Each period of ZT 0–20 represents a distinct biological replicate. (c) Bars heights represent normalized counts of LLCs and genes rhythmic in young or old, which peak at indicated 4-h intervals (Methods). (d) qRT-PCR expression of LLCs in heads of females at age 5, 55 and 75 days. (e) qRT-PCR expression of LLCs in heads of young and old males. (d,e) Data are mean (from two cycles)±s.e.m. Expression is per cent of peak expression in the oldest group. *P≤0.05; **P≤0.01; ***P≤0.001; ****P≤0.0001 (two-way ANOVA with Bonferroni correction; day 55 change is relative to day 5 values, and day 75 change is relative to day 55 values).
