Figure 5: Age-induced circadian expression of novel genes. | Nature Communications

Figure 5: Age-induced circadian expression of novel genes.

From: Circadian deep sequencing reveals stress-response genes that adopt robust rhythmic expression during aging

Figure 5

(a) RNA-seq expression plots for the five LLC-like novel genes; each period of ZT 0–20 represents a distinct biological replicate. XLOC IDs were assigned by Cuffmerge. (b) Browser image of the crescendo transcript model and corresponding RNA-seq reads from merged replicate datasets of old flies, with the same y-axis range across time points. The dm6 phastCons conservation tracks were downloaded from the UCSC Genome Browser website. (c) crescendo expression in heads of females on day 5, 55 and 75 by qRT-PCR is reported as per cent of peak level at day 75. Data are mean (from two cycles)±s.e.m. *P≤0.05; **P≤0.01; ***P≤0.001; ****P≤0.0001 (two-way ANOVA with Bonferroni correction; day 55 change is relative to day 5 values, and day 75 change is relative to day 55 values).

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