Figure 3: Cx3cr1-deficient female mice display HFD-induced hypothalamic microglial accumulation and activation.
From: Sex differences in microglial CX3CR1 signalling determine obesity susceptibility in mice
(a–d,f–i) Representative images showing Iba1 immunoreactivity in the MBH of male (a–d) and female mice (f–i). 3V=third ventricle. (e,j) Quantification of Iba1-positive cells in bilateral MBH from six sections per animal. Mean±s.e.m., n=4 per group. Scale bar, 50 μm. (k,l) Representative images showing microglial morphology in the MBH of HT and KO females. Scale bar, 10 μm. (m) Quantification of total dendritic length in MBH from 10 individual microglial cells per animal. Mean±s.e.m., n=4 per group. (n) mRNA levels of inflammatory markers in isolated hypothalamic microglia following 12-week HFD exposure in HT and KO female mice. Data are presented as per cent change relative to HT-chow controls. Mean±s.e.m., n=5–7 per group. For all panels, data are analysed by two-way ANOVA followed by Bonferroni post hoc comparisons. *P<0.05.
