Figure 4: Optogenetic activation of layer II/III and layer V/VI pyramidal neurons in vivo. | Nature Communications

Figure 4: Optogenetic activation of layer II/III and layer V/VI pyramidal neurons in vivo.

From: Layer-specific optogenetic activation of pyramidal neurons causes beta–gamma entrainment of neonatal networks

Figure 4

(a) Representative raster plot and corresponding spike probability histogram displaying the firing of transfected layer II/III pyramidal neurons in response to 30 sweeps of ramp illumination (473 nm, 3 s). (b) Left, bar diagram displaying the mean MUA frequency in ChR2(ET/TC)-transfected neurons from P8 to P10 mice in response to ramp illumination. Right, occurrence rate of interspike intervals averaged for 3 s before light stimulation (pre, black), 3 s during ramp stimulation (stimulation, blue) and 3 s after light stimulation (post, grey, n=21 recording sites from 9 pups). Note the prominent increase in the rate of interspike intervals between 12 and 30 Hz peaking at 16.7 Hz during ramp stimulation. (c) Same as (b) for SUA (n=50 units from 9 pups). Note that the peak between 12 and 30 Hz is present, but less pronounced due to the incorporation of non-triggered units. (d–f) Same as (a–c) for layer V/VI pyramidal neurons (red, n=12 pups; MUA of n=61 recording sites; SUA of n=152 units). For (b,c,e,f) grey lines correspond to firing of individual neurons. Data are presented as mean±s.e.m. *P<0.05, **P<0.01 and ***P<0.001), one-way repeated-measures analysis of variance (ANOVA) with Bonferoni-corrected post hoc analysis.

Back to article page