Figure 5: The Quintet-MFP supported the integration of female reproductive tissues.
From: A microfluidic culture model of the human reproductive tract and 28-day menstrual cycle
(a) Concentrations of pituitary hormones, FSH and hCG, during 28-day culture in the Quintet-MFP. (b,c) Ovarian hormone secretion during 28-day microfluidic culture with oestradiol (E2) and progesterone (P4) in b and inhibin A and inhibin B in c. (d) Dynamic E2 concentrations in the donor and acceptor modules over 48 h. (e) Images of fallopian epithelium histology, cilia image and OVGP1 expression on days 0 and 7 (14 and 21 days of microfluidic culture) in the Quintet-MFP. (f) Human endometrium tissue before and after decellularization, after recellularization, and immunohistochemistry staining of vimentin, cytokeratin, Ki67, ER and, PR on day 14 (28 days of microfluidic culture) in the Quintet-MFP. Graphs of a–d display average+s.d. Scale bar, 100 μm for fallopian epithelium histology, 10 μm for cilia images (e), and 50 μm for endometrium images (f). OVGP1 expression: α-tubulin as internal control. n=3 replicates of the integrated tissue culture in the Quintet-MFP.
