Figure 1: Live-cell transduction ability of CPPs and experimental and theoretical shape parameters. | Nature Communications

Figure 1: Live-cell transduction ability of CPPs and experimental and theoretical shape parameters.

From: Backbone rigidity and static presentation of guanidinium groups increases cellular uptake of arginine-rich cell-penetrating peptides

Figure 1

(a) The CPPs PTD4, TAT, R10 and cyclic TAT were transduced, or otherwise internalized, into living mouse myoblast cells in the presence of PBS. Representative transmission images of the phase contrast and confocal optical sections of the fluorescent peptides are shown. Scalebars, 10 μm. Arrows indicate the presence of fluorescent peptide inside nucleoli and percentages of the number of transduced cells are given. (b) Results from the sedimentation velocity ultracentrifugation experiments and subsequent analysis: f/f0: frictional ratio (squares), d: diameter, end–end distances (circles). (c) Results of the molecular dynamics simulations: radius of gyration (squares) and end–end distances (circles). Q* results from a decondensation reaction between a glutamic acid and a lysine during cyclization (see Supplementary Table 1).

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