Figure 2: Kinetic uptake characteristics of a linear and cyclic form of TAT.

(a) Confocal optical sections of timelapse movies (Supplementary Movie 1) show the uptake of fluorescently labelled linear and cyclic TAT in the presence of serum-free medium. Scalebar, 10 μm. (b) Mean fluorescence intensity of fluorescein-labelled linear (triangles) and cyclic (circles) TAT inside the nucleus was monitored over 60 min at a frame rate of one image every 79 s. (c) Radii of gyration (circles) and guanidinium group distances (squares) derived from the molecular dynamics simulations.