Figure 3: Kinetic uptake characteristics of a linear and cyclic form of R10. | Nature Communications

Figure 3: Kinetic uptake characteristics of a linear and cyclic form of R10.

From: Backbone rigidity and static presentation of guanidinium groups increases cellular uptake of arginine-rich cell-penetrating peptides

Figure 3

(a) Confocal optical sections of timelapse movies (Supplementary Movie 6) show the uptake of fluorescently labelled linear and cyclic R10 in the presence of serum-free medium. Scalebar, 10 μm. Because of the much higher cellular uptake of these peptides, the contrast adjustment needed does not allow visualization of the peptide in the extracellular media as in the TAT case (Fig. 2a). (b) Mean fluorescence intensity of fluorescein-labelled linear (triangles) and cyclic (circles) R10 inside the nucleus was monitored over 60 min at a frame rate of one image every 60 s.

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