Figure 5: Differential expression of PSD proteins involved in intracellular vesicle biogenesis between mouse and zebrafish.

For each protein the mouse orthologue gene name is given. Proteomic data are provided for both species. For proteomic data, a white square denotes that we did not detect that protein, a blue square identifies proteins found in synaptosomes but not in PSDs and a red square identifies proteins found at the PSD. The average mRNA expression (mean transcripts per million (TPM) from four whole-brain biological replicates) was determined for each gene (for TPMs of individual samples, see Supplementary Fig. 10). For mRNA-sequencing data, a white box denotes a mean expression of <1 TPM, a pale green box <10 TPM and dark green >10 TPM. (a) Proteins involved in vesicle fusion with membranes, including SNARE complex proteins: Syntaxins, Vamps and SNAPs, syntaxin-binding proteins (Sec1/Munc18) and Synaptotagmins. (b) Proteins form the HOPS complex involved in membrane vesicle tethering to membranes. (c) Proteins involved in membrane bending/budding and scission to form vesicles, including proteins from ESCRT complexes (ESCRT I, II, III and Vps4-Vta1/ALIX), Dynamins and small GTPases from the Rab family.