Figure 5: JR-CSF HIV-1 challenge of humanized mice treated with VRC01 mRNA-LNPs. | Nature Communications

Figure 5: JR-CSF HIV-1 challenge of humanized mice treated with VRC01 mRNA-LNPs.

From: Administration of nucleoside-modified mRNA encoding broadly neutralizing antibody protects humanized mice from HIV-1 challenge

Figure 5

CD34-NSG humanized mice were i.v. injected with 30 μg (1 mg kg−1) of VRC01 or 30 μg of Luc mRNA-LNPs. (a) Animals were bled 24 h post-injection and VRC01 levels were measured in the plasma by ELISA. (b) Viral loads (RNA copies per 10 μl plasma) at 1 and 2 weeks post-challenge were measured by qRT–PCR. (c) Gag DNA integration was measured in splenocytes by conventional PCR 3 weeks post challenge. Ultraviolet-illuminated ethidium bromide-stained PCR products are shown. The pNL4-3 integration standard was included to determine the sensitivity of the PCR assay. Error bars are s.e.m. Student’s t-test, two-sided, comparing Luc treated to VRC01 treated were highly significant: (a) P<0.001, (b) P<0.001. Group size is four animals.

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