Figure 4: Atg2 overexpression corrects LD size in M. marinum-infected cells.
From: Regulation of phagocyte triglyceride by a STAT-ATG2 pathway controls mycobacterial infection
(a) Intracellular neutral lipid in M. marinum-infected cells overexpressing either HA (control) or Atg2. Scale bars, 5 μm. (b) Quantification of neutral lipid volume per cell and total number of LDs with at least one axis >1 μm in length in cells treated as in a. A slight increase in the overall volume of neutral lipids was observed in Atg2-overexpressing cells compared with control (*P<0.05 and **P<0.01, Mann–Whitney test, n=130 for non-infected controls, n=110 for Mm-infected controls, n=107 for non-infected cells overexpressing Atg2 and n=100 for infected cells overexpressing Atg2). (c) Distribution of large (>1.5 μm3) LD volumes in cells overexpressing Atg2 or HA and with the infections indicated. Volumes <1.5 μm3 are not shown but were included in statistical analysis. Punctae (3,100–5,000) were analysed depending on genotype and condition. Significance values are from the Kolmogorov–Smirnov test. (d) Distribution of large (>1.5 μm3) LD volumes in cells with Luciferase or Stat92E knocked down by RNAi, with or without infection as indicated. Volumes <1.5 μm3 are not shown but were included in statistical analysis. Punctae (1,100 to 2,000) were analysed depending on genotype and condition. Significance values are from the Kolmogorov–Smirnov test.
