Figure 1: Deletion of SWI/SNF subunits results in H3K27ac loss and enhancer deactivation. | Nature Communications

Figure 1: Deletion of SWI/SNF subunits results in H3K27ac loss and enhancer deactivation.

From: The SWI/SNF chromatin remodelling complex is required for maintenance of lineage specific enhancers

Figure 1

(a) Western blots of selected factors, histones and histone modifications in control MEFs (-CRE) and MEFs with CRE-inactivated SWI/SNF (Arid1a, Smarca4, Smarcb1) or Polycomb (Ezh2) subunits. (b). H3K27ac signal at promoters and enhancers in Smarcb1-deficient or Smarca4-deficient versus wild-type MEFs. (c). Representative screenshot showing localization of subunits SMARCC1 and SMARCA4 and histone marks H3K4me3, H3K4me1 and H3K27Ac in wild-type, Smarcb1-deficient and Smarca4-deficient MEFs showing lost enhancers. (d) Log-fold changes of histone modifications H3K4me1 versus H3K27ac at promoters and enhancers in Smarcb1-deficient or Smarca4-deficient relative to wild-type MEFs.

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