Figure 2: FBXW7 inhibits RNA virus infection and promotes type-I IFN production in vitro.
From: E3 ligase FBXW7 is critical for RIG-I stabilization during antiviral responses
(a–b) Quantitative PCR (Q-PCR) analysis of IFN-β, IFN-α4 mRNA expression in FBXW7f/f and Lysm+FBXW7f/f peritoneal macrophages (a) or BMDC (b) infected with VSV, H1N1 virus or RSV. (c) ELISA assay of IFN-β in supernatants of FBXW7f/f and Lysm+FBXW7f/f peritoneal macrophages or BMDC infected for 24 h with VSV, H1N1 virus or RSV. (d) Q-PCR analysis of ISG15 and ISG20 mRNA expression in FBXW7f/f and Lysm+FBXW7f/f peritoneal macrophages infected with VSV, H1N1 virus or RSV. (e–f) Q-PCR analysis of VSV-G, Flu A and RSV-G mRNA expression in FBXW7f/f and Lysm+FBXW7f/f peritoneal macrophages (e) or BMDC (f) infected with VSV, H1N1 or RSV for 12 h. (g,h) Flow cytometry analysis of GFP fluorescence intensity in FBXW7f/f and Lysm+FBXW7f/f peritoneal macrophages (g) or BMDC (h) infected with VSV-GFP. Data are mean±s.e.m. and are representative of three independent experiments. Student’s t-test was used for statistical calculation. *P<0.05, **P<0.01 and ***P<0.001.
