Figure 4: BMT ameliorates impaired cardiac function after MI in EP3 KO mice.

(a) Bone marrow transplantation (BMT) between Ep3 KO and WT mice was confirmed by genotyping. (b–d) Recruitment of total Mos/Mps (b), Ly6C^high (c) and Ly6C^low (d) Mos/Mps in injured hearts in chimeric mice that underwent BMT. Data represent mean±s.e.m. *P<0.05 as indicated (unpaired two-tailed t-test); n=6–7. (e) mRNA expression levels of VEGF, FGF, HGF, PDGFbb and CX3CR1 in Mos/Mps sorted from hearts from BMT chimeric mice at day 14 post MI. Data represent mean±s.e.m. *P<0.05 as indicated (unpaired two-tailed t-test);n=4. (f) Representative immunostaining of CD31 (red) and PCNA (green) in peri-infarct zones of hearts from BMT chimeric mice at day 14 post MI. The solid box outlines the region enlarged to the right. Yellow arrow, CD31⁺/PCNA⁺ cells. Scale bar, 20 μm. (g,h) Quantitation of CD31⁺ areas (g) and PCNA⁺CD31⁺ cells (h) in injured hearts as shown in f. Data represent mean±s.e.m. *P<0.05 as indicated (unpaired two-tailed t-test); n=6–7. (i) Cardiac function of BMT chimeric mice at day 14 after MI. EF, ejection fraction. Data represent mean±s.e.m. *P<0.05 as indicated (unpaired two-tailed t-test); n=8–11.