Figure 5: MAX restoration also restores p16 expression and function in GIST.
From: MAX inactivation is an early event in GIST development that regulates p16 and cell proliferation
MAX restoration in MAX-mutant (homozygous deletion) GIST48 cell line restores p16 expression and inhibits CDK4/6-dependent phospho-RB1Ser795; GAPDH serves as loading control and the bar graph normalizations of GIST48 versus GIST48MAX are with the higher value set to 100% (a). Restoration of MAX expression inhibits GIST48 growth, as assessed by cell counts at 24 h (P=0.0488) and 72 h (P=0.0029) (b), and inhibits GIST proliferation index, as assessed by BrdU incorporation at 24 h (P=0.0130) (Student’s t-test) (c). Tests were performed in triplicate. The error bars show s.d. (b) and s.e.m. (c).
