Figure 3: Molecular signature of the proliferative hub and the LE during WH.
From: Defining stem cell dynamics and migration during wound healing in mouse skin epidermis
(a) Scheme showing the strategy used to isolate the LE with a 4 mm punch biopsy and the proliferative hub with a 6-mm-punch biopsy. (b) Representative maximum intensity projection of confocal pictures showing the immunostaining of whole-mount epidermis with α5-integrin (red) and anti-BrdU (green) 0, 2 and 4 days after wound. Note the expression of the α5-integrin by the non-proliferative LE cells only. (c) Gene ontology enrichment in the LE 4 days after wound (n=3). (d–j) LE signature. List of genes upregulated in the α5-integrin positive cells of the LE compared with the proliferative hub (n=3). These genes are implicated in cell migration (d), inflammation (e), cell adhesion (f), transcription (g), ECM composition (h), cytoskeleton and actin regulators (i) and cell signalling (j).
