Figure 2: Genetically modified human ductal cells develop PanIN-like lesions after orthotopic transplantation. | Nature Communications

Figure 2: Genetically modified human ductal cells develop PanIN-like lesions after orthotopic transplantation.

From: Reconstituting development of pancreatic intraepithelial neoplasia from primary human pancreas duct cells

Figure 2

(a) Schematic diagram of the orthotopic transplantation procedure. (b) immunohistochemical analyses of a PanIN-like structure in transplanted animal ID 193 with the human nuclei-specific antibody (HuNu, white) and H2B-mCherry fluorescence (red) along with 4,6-diamidino-2-phenylindole (DAPI) nuclear staining (blue). (c) Haematoxylin and eosin (H&E) staining of the PanIN-like structures found in transplanted animal ID 192 with magnified view of red-boxed area in d. (e) Alcian blue staining of tissue section adjacent to that shown in d. (f) Immunohistochemical analysis with phospho-ERK antibody. (g) H&E staining of the transplanted mouse ID 194 with magnified view of the red-boxed area in h. (i) Alcian blue staining of tissue section adjacent to that shown in h. (j) Immunohistochemical analysis with phospho-ERK antibody. Scale bars, 100 μm.

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