Figure 5: Clones with defined genomic mutations form PanIN-like lesions but not PDA. | Nature Communications

Figure 5: Clones with defined genomic mutations form PanIN-like lesions but not PDA.

From: Reconstituting development of pancreatic intraepithelial neoplasia from primary human pancreas duct cells

Figure 5

(a) Schematic of the sphere clone isolation procedure. See Methods for details. (b) Genomic DNA PCR confirming the presence of lentiviral transgenes in clone 3 (left) and clone 4 (right). (c) Relative mRNA expression level of oncogenic KRAS transgene (bottom) and the transgene plus endogenous KRAS (top) of clone 3 (left) and clone 4 (right). Error bars=s.d.; n=2. (d,e) H&E (left) and Alcian blue (right) staining of the transplanted pancreas ID 199 with clone 3 in d and ID 207 with clone 4 in e. Yellow arrows indicate representative abnormal nuclei, red and green arrows indicate necrotic cells and epithelial cell clusters found in the lumen, features of human PanIN2 and 3. Scale bars, 200 μm.

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