Figure 6: Genetic modification of human ductal cell line HPDE induces invasive PDA development. | Nature Communications

Figure 6: Genetic modification of human ductal cell line HPDE induces invasive PDA development.

From: Reconstituting development of pancreatic intraepithelial neoplasia from primary human pancreas duct cells

Figure 6

(a) Genomic DNA PCR for assessing the presence of lentiviral transgenes in HPDE cells. (b) Relative mRNA expression level of oncogenic KRAS and ERBB2 transgene. Error bars=s.d.; n=2. (c) Indel efficiency of each indicated genomic locus assessed by TIDE analysis. (d) Stereoscopic and representative haematoxylin and eosin (H&E) and anti-CK19 (green) immunostaining images of the tumours formed in the transplanted pancreas with HPDEKECST. Red arrows indicate tumour nodules. (e) Representative H&E staining of lung with metastatic cells found in ID 211. The metastatic cells are CK19+ (green, bottom). (f) Stereoscopic and representative H&E staining images of the tumours formed in the transplanted pancreas with HPDEKCST. Scale bars, 200 μm.

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