Figure 7: Glutathione supplementation protects hepatocytes from DEN-induced DNA damage and apoptosis. | Nature Communications

Figure 7: Glutathione supplementation protects hepatocytes from DEN-induced DNA damage and apoptosis.

From: Inhibition of hepatic lipogenesis enhances liver tumorigenesis by increasing antioxidant defence and promoting cell survival

Figure 7

Viability of primary hepatocytes treated with (a) DEN or (b) tBuOOH for 24 h. Representative images and quantification of immunofluorescence in primary hepatocytes treated with vehicle (Mock) or DEN for 24 h, or pretreated with GEE (1 mM) for 1 h prior to DEN treatment, probed with primary antibodies against (c) yH2A.X and (d) cleaved CASP3. Nuclei were counter-stained with 4,6-diamidino-2-phenylindole. Scale bar, 100 μm. Quantification for a–d includes three and two independent experiments, respectively. * Indicates significant difference, P<0.05 as determined by one-way analysis of variance followed by Tukey’s post hoc analyses. Data are represented as mean±s.e.m.

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