Figure 8: Single-point mutations in FRET reporters.
From: Structure-guided mutagenesis reveals a hierarchical mechanism of Parkin activation
(a,b) FRET images and average FRET efficiency of WT and activating point mutations N273K, W403A and F146A in the FRET-380 and FRET-81 reporters. (c) Quantification of FRET efficiency measurements from a,b. Each data point represents the FRET efficiency measurement from one cell. (d) FRET efficiency measurements and (e) quantification of the S65E phosphomimetic in the FRET-380 reporter. Vertical bars represent s.e.m. for three independent experiments. ***P<0.0001; n.s., not significant (one-way ANOVA with Tukey's post test). Scale bars, 20 μm.
