Figure 3: MCT2 is required for the enhancement of tumorigenic activity associated with MGDAs. | Nature Communications

Figure 3: MCT2 is required for the enhancement of tumorigenic activity associated with MGDAs.

From: Adipocytes promote malignant growth of breast tumours with monocarboxylate transporter 2 expression via β-hydroxybutyrate

Figure 3

(a) Q-PCR and western blot analyses of ARMCX1, ENPP1 and MCT2 depletion and ectopic overexpression in breast cancer cells. (b) Soft agar colony formation assays showed that depletion of ARMCX1, ENPP1 and MCT2 abrogated the increase of colonies induced by MGDAs co-culture in MCF7 and MDA-MB-231 cells. (c) Soft agar colony formation assays of ARMCX1, ENPP1 and MCT2 overexpressing MDA-MB-468 and SK-BR3 cells w/ or w/o MGDAs co-culture. (d) Tumour growth assays in NOD/SCID/γnull mice. MCT2-depleted MDA-MB-231 and overexpressing MDA-MB-468 cells were subcutaneously injected in both flanks of each mouse w/ or w/o MGDAs. Tumour volumes were measured every 7 days. Four mice (n=4) were used for each group. (e). Tumour weights were increased in the presence of MCT2 after co-injection with MGDAs in mouse tumorigenicity assays. The experiments in b,c were performed in technical triplicate and repeated at least twice with similar results. In b–e, data show means±s.d. *P<0.05 (Student’s t-test).

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