Figure 2: Nerve injury-induced increases in Dnmt3a mRNA and DNMT3a protein levels in the injured DRG. | Nature Communications

Figure 2: Nerve injury-induced increases in Dnmt3a mRNA and DNMT3a protein levels in the injured DRG.

From: DNA methyltransferase DNMT3a contributes to neuropathic pain by repressing Kcna2 in primary afferent neurons

Figure 2

(a) DNMT3a and DNMT3b protein expression in the ipsilateral L5 DRG after SNL or sham surgery. n=6 rats/time point. One-way ANOVA followed by post hoc Tukey test, Ftime (3, 15)=8.09 for DNMT3a and Ftime (3, 15)=0.07 for DNMT3b. *P<0.05 versus the corresponding control group (0 day). Full-length blots are presented in Supplementary Fig. 6. (b,c) Neurons labelled by DNMT3a and NeuN in the ipsilateral L5 DRG on days 3 (c) and 7 (b,c) after SNL or sham surgery. n=5 rats/time point/group. **P<0.01 versus the corresponding sham group by two-tailed unpaired Student’s t-test. Scale bar: 50 μm. (d) Dnmt3a mRNA expression in the ipsilateral L5 DRG on days 0, 3, and 7 after SNL or sham surgery. n=6 rats/time point/group. Two-way ANOVA followed by post hoc Tukey test, Ftime (2, 48)=7.4. *P<0.05 versus the corresponding control group (0 day), (e,f) DNMT3a and DNMT3b proteins (e) and their mRNAs (f) in the ipsilateral L4 and L5 DRG on day 7 after CCI or sham surgery. n=6 rats/group. *P<0.05 or **P<0.01 versus the corresponding sham group by two-tailed unpaired Student’s t-test. Full-length blots are presented in Supplementary Fig. 6.

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