Figure 6: DRG Dnmt3a overexpression produces neuropathic pain symptoms in both rats and mice. | Nature Communications

Figure 6: DRG Dnmt3a overexpression produces neuropathic pain symptoms in both rats and mice.

From: DNA methyltransferase DNMT3a contributes to neuropathic pain by repressing Kcna2 in primary afferent neurons

Figure 6

GFP: AAV5-GFP in a–e or HSV-GFP in f–j. DNMT3a: AAV5-Dnmt3a in a–e or HSV-Dnmt3a in f–j. (a) Amounts of Dnmt3a, Dnmt1, and Dnmt3b mRNAs in the ipsilateral and contralateral L4/5 DRG 8 weeks after viral microinjection into the ipsilateral L4/5 DRG in rats. n=3 rats per group. **P<0.01 versus the corresponding GFP group by two-tailed unpaired Student’s t-test. (b) Levels of DNMT3a and DNMT1 proteins in the ipsilateral L4/5 DRG in naive rats and 8 weeks after viral microinjection into the ipsilateral L4/5 DRG in rats. n=3 rats/group. One-way ANOVA followed by post hoc Tukey test, Fgroup (2, 8)=40.7 for DNMT3a and Fgroup (2, 8)=1.21 for DNMT1. **P<0.01 versus naive rats. Full-length blots are presented in Supplementary Fig. 6. (c–e) Paw withdrawal responses to mechanical (c), thermal (d) and cold (e) stimuli on the ipsilateral (ipsi) and contralateral (contral) sides at time points as shown after viral microinjection into the ipsilateral L4/5 DRG in rats. n=10 rats per group. Two-way ANOVA followed by post hoc Tukey test, Fgroup (3, 119)=206.9 for (c), Fgroup (3, 119)=69.1 for (d), and Fgroup (1, 59)=178.0 for (e). **P<0.01 versus the corresponding GFP group on the ipsilateral side. (f–i) Ipsilateral paw withdrawal responses to mechanical (f,g), thermal (h) and cold (i) stimuli at time points as shown after viral microinjection into the ipsilateral L3/4 DRG in mice. n=10 mice per group. Two-way ANOVA followed by post hoc Tukey test, Fgroup (1, 23)=88.2 for (f), Fgroup (1, 23)=28.4 for (g), Fgroup (1, 23)=25.2 for (h) and Fgroup (1, 23)=37.8 for (i). *P<0.05 or **P<0.01 versus the corresponding GFP group. (j) Levels of DNMT3a, p-ERK1/2, ERK1/2 and GFAP in the ipsilateral L3/4 dorsal horn 6 days after viral microinjection into the ipsilateral L3/4 DRG in mice. n=12 mice per group. *P<0.05 versus the corresponding HSV-GFP group by two-tailed unpaired Student’s t-test. Full-length blots are presented in Supplementary Fig. 6.

Back to article page