Figure 7: Blocking DRG DNMT3a increase rescues Kcna2 expression in the injured DRG of rats or mice post-SNL. | Nature Communications

Figure 7: Blocking DRG DNMT3a increase rescues Kcna2 expression in the injured DRG of rats or mice post-SNL.

From: DNA methyltransferase DNMT3a contributes to neuropathic pain by repressing Kcna2 in primary afferent neurons

Figure 7

(a,b) Levels of Kcna1, Kcna2, and Kcna4 mRNAs in the ipsilateral (Ipsi) or contralateral (Contral) L5 DRG (a) and levels of Kcna1 and Kcna2 proteins in the ipsilateral L5 DRG (b) from the AAV5-Dnmt3a shRNA (shRNA)- or AAV5-Dnmt3a scrambled shRNA (scram)-injected rats on day 7 post-SNL or sham surgery. n=6 rats/group. One-way ANOVA followed by post hoc Tukey test, Fgroup (3, 15)=18.9 for Kcna1 mRNA, Fgroup (3, 15)=128.6 for Kcna2 mRNA, Fgroup (3, 15)=38.9 for Kcna4 mRNA, Fgroup (2, 8)=0.02 for Kcna1 protein, and Fgroup (2, 8)=12.2 for Kcna2 protein. *P<0.05 or **P<0.01 versus the corresponding sham plus scram group. #P<0.05 or ##P<0.01 versus the corresponding SNL plus scram group. Full-length blots are presented in Supplementary Fig. 6. (c,d) Levels of Kcna1, Kcna2 and Kcna4 mRNAs in the ipsilateral (Ipsi) or contralateral (Contral) L4 DRG (c) and levels of Kcna2 and Kcna4 proteins in the ipsilateral L4 DRG (d) from the AAV5-Cre (Cre)- or AAV5-GFP (GFP)-injected Dnmt3afl/fl mice on day 7 post-SNL or sham surgery. n=12 mice/group. One-way ANOVA followed by post hoc Tukey test, Fgroup (4, 16)=9.68 for Kcna1 mRNA, Fgroup (4, 16)=36.9 for Kcna2 mRNA, Fgroup (4, 16)=6.40 for Kcna4 mRNA, Fgroup (2, 8)=13.4 for Kcna2 protein, and Fgroup (2, 8)=0.10 for Kcna4 protein. *P<0.05 or **P<0.01 versus the corresponding sham plus GFP group. ##P<0.01 versus the corresponding SNL plus GFP group. Full-length blots are presented in Supplementary Fig. 6.

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