Figure 1: Expression of Orai1 and Orai2 genes in T cells.
From: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity
(a,b) LacZ expression (Orai2 reporter) in thymic and peripheral T cell populations of Orai2LacZ/+ and WT mice. (a) Detection of the green fluorescent LacZ substrate FDG in T cells from WT and Orai2LacZ/+ mice by flow cytometry. (b) Quantification of normalized FDG fluorescence intensity (MFIOrai2−MFIWT) in thymocytes and splenic T cell populations as shown in a; means±s.e.m. of three mice. DN: CD4−CD8− double negative; DP: CD4+CD8+ double positive; CD4SP: CD4+ single positive; CD8SP: CD8+ single positive. (c) Expression of Orai1, Orai2 and Orai3 genes in FACS-sorted CD4+CD62LhiCD44lo naive (Tnaive) and CD4+CD62LloCD44hi effector T cells (Teff) analysed by qRT–PCR; means±s.e.m. of four mice. (d) Ratio of Orai1 to Orai2 gene expression in FACS-sorted Tnaive and Teff cells; means±s.e.m. of four mice. (e) Ratio of Orai1 to Orai2 gene expression following anti-CD3/CD28 stimulation of murine naive CD4+ T cells in vitro; means±s.e.m. of three mice. *P<0.05; **P<0.005; ***P<0.001 in (c–e) using unpaired Student’s t-test.
