Figure 7: Humoral immunity requires both ORAI1 and ORAI2 in TFH cells.
From: ORAI2 modulates store-operated calcium entry and T cell-mediated immunity
(a,b) Analysis of CD4+CXCR5hiPD-1hi TFH cells (a) and CD19+CD38−GL.7+ GC B cells (b) in spleens of WT, Orai1fl/flCd4cre, Orai2−/− and Orai1fl/flOrai2−/−Cd4cre (DKO) mice 10 days after LCMV infection by flow cytometry; means±s.e.m. of four to eight mice. (c) Representative immunofluorescence of GCs in WT, Orai1fl/flCd4cre, Orai2−/− and DKO mice 10 days after LCMV infection; PNA (green) and B220 (red). Scale bar, 100 μm. (d) Analysis of LCMV-specific IgM and IgG levels in the sera of WT, Orai1fl/flCd4cre, Orai2−/− and DKO mice 10 days after LCMV infection; means±s.e.m. of four to six mice. *P<0.05; **P<0.005; ***P<0.001 in (a,b) using unpaired Student’s t-tests.
