Figure 4: Crinkler gene-family relationship and expression patterns.
From: Genomic innovations linked to infection strategies across emerging pathogenic chytrid fungi
(a) A phylogenetic tree inferred using RAxML from the N-terminal region (first 50 amino acids) of all identified CRNs, showing Bd genes in green, Bsal genes in red, Sp genes in blue and Hp genes in black (branch lengths indicate the mean number of nucleotide substitutions per site). Two motifs were identified from these sequences. (b) Most CRNs were downregulated in Tw, and all those that were significantly differentially expressed (shown) were downregulated, including three Bsal genes (indicated by asterisk). Trees indicate hierarchical clustering between data sets (above) and genes (left of heatmap). (c) CRN mean fold changes in mRNA expression profile in Bd (above) and Bsal (below). The data show the normalized target gene amount in spores that were incubated with skin tissue of Tw for 2 h, 3-day-old sporangia grown in TGhL and skin tissue from chytrid-infected Tw animals relative to freshly collected spores which is considered 1. Significant differences in expression between each experimental group are shown in Supplementary Table 6.
