Figure 6: Neuronal smg-2 plays a major role in the long lifespan of daf-2 mutants.

(a–d) Effects of neuron- (a), hypodermis- (b), body wall muscle- (c) and intestine- (d) specific RNAi knockdown of smg-2 on the longevity of daf-2(−) animals. See Supplementary Fig. 11 for the lifespan graphs of control and another hypodermis-specific RNAi strain. (e–h) The lifespan curves with transgenic animals expressing smg-2::gfp in neurons (e), the hypodermis (f), muscles (g) and the intestine (h) in smg-2(qd101); daf-2(e1370) (smg-2(−); daf-2(−)) mutants. See Supplementary Data 1 for statistical analysis and additional repeats. (i,j) Analysis of PTC- (i) or uORF- (j) containing transcripts among transcripts downregulated (Down: log₂(fold change)−1, P0.1) in the neurons of daf-2(−) mutants compared to those of WT²⁴ (*P<0.05, **P<0.01, χ²-test). See Supplementary Table 1 for actual P values. (k) Box plots showing fold changes of neuronal transcripts in daf-2 mutants compared to those in WT, categorized by different 3′ UTR lengths. Long (1,500 nt), medium (350 nt<<1,500 nt ) and short (350 nt). 3′ UTR lengths were defined following a previous report⁵⁰. Thick black lines indicate median values. Bottom and top of the box plot represent 25th and 75th percentile, respectively, and whiskers indicate the data within the 1.5 interquartile range, which is the distance between the lower and upper quartiles of the data (**P<0.01, ***P<0.001, Wilcox rank-sum test).