Figure 1: Plasmonic gold-on-gold nano-island films enhance the fluorescence of near-infrared fluorophores. | Nature Communications

Figure 1: Plasmonic gold-on-gold nano-island films enhance the fluorescence of near-infrared fluorophores.

From: Plasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range

Figure 1

(a) Scanning electron micrograph (scale bar, 1 μm) of a solution-phase grown Au/Au film on glass showing the nanoscopic gold island morphology. Inset shows digital photograph of an Au/Au film-coated glass slide with scale bar, 1 cm. (b) Extinction spectrum of Au/Au film overlaid with the excitation (line) and emission (shaded area) regions of Cy5 and IR800 dyes, respectively. (c) Fluorescence intensity maps of IgG–Cy5 (top) and IgG-IR800 (bottom) conjugates drop dried onto glass or the Au/Au film from (a). Images for each fluorophore on glass and Au/Au are shown on the same intensity scale for comparison. Analyte spot size is ∼2 mm in diameter. (d) Fluorescence emission spectra of IgG-bound Cy5 (left) and IR800 (right) dried onto the Au/Au film (shown in green and blue, respectively) from (a) as well as glass for comparison (shown in black).

Back to article page