Figure 2: Near-infrared fluorescence enhanced protein microarrays on gold substrates probed by IR800. | Nature Communications

Figure 2: Near-infrared fluorescence enhanced protein microarrays on gold substrates probed by IR800.

From: Plasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range

Figure 2

(a) Top panel: μArray/Au fluorescence maps generated by integration of goat anti-rabbit IgG-IR800 fluorescence emission at 785 nm excitation for different concentrations (12 duplicate spots for each concentration) of the analyte, CEA spiked into whole, undiluted serum. Lower panel: fluorescence maps on the same intensity scale as the top panel for comparison, generated in an identical fashion on a glass substrate (inset shows fluorescence maps with intensity increased by ×5). (b) Calibration curves for CEA quantification were generated by averaging the integrated fluorescence intensity of goat anti-rabbit IgG-IR800 emission over the 12 duplicate microarray spots for each CEA concentration on a μArray/Au assay as well as a protein microarray on glass as shown in schematics. Error bars represent the standard deviation of the mean over the 12 duplicate assay features.

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