Figure 2: Functional analysis of CTB4a at the booting stage.
From: Natural variation in CTB4a enhances rice adaptation to cold habitats
(a) Schematic of vectors for transgenic analysis. C, complementation vector; K-OE, CTB4aKMXBG overexpression vector; T-OE, CTB4aTowada overexpression vector. (b) Statistical results for seed setting of Towada, NIL1913, C11 and C12 under cold stress. Data represent means±s.d. (n=20). (c) Statistical results for seed setting of Towada and overexpression lines under cold stress. Data represent mean±s.d. (n=25). (d) Phenotype of plants and panicles of overexpression lines grown under CS-HAA. (e) Statistical results for seed setting of Nip and RNAi lines under cold stress. Data represent means±s.d. (n=15). (f) Phenotype of plants and panicles of RNAi lines grown under CS-HAA. (g) Phenotypic analysis of HY and ctb4a grown under CS-PT and CS-DW. Data represent means±s.d. (n=10). (h) Panicles of HY and ctb4a mutant under CS-PT. The presence of the same lowercase letter above the error bar denotes a non-significant difference between the means (P>0.05, Student’s t-test).
