Figure 3: Association of IPA1 and microRNA expression with changes in IM development.
From: A natural tandem array alleviates epigenetic repression of IPA1 and leads to superior yielding rice
(a) Relative expression of IPA1 in NILipa1-2D and NILIPA1, normalized to the rice Actin gene, in various organs including seedlings (S), root (R), mature leaf (L), different stages of IM (IM1–IM6), young panicle (YP) and flowering panicle (P). Values are means±s.d. (n=3). (b,c) Relative expression of miRNA156 (b) and miRNA529 (c) in various organs of NILs, normalized to rice 5 S rRNA. Values are means±s.d. (n=3). (d,e) Comparison of IM circumference (d) and area (e) between NILs at the IM1 stage in a. Values are means±s.d. (n=8). Triple asterisks represent significant difference between NILs determined by the Student’s t-test at P<0.001. (f,g) Scanning electron microscope images of IMs in NILipa1-2D (f) and NILIPA1 (g) at the IM1 stage in a. Scale bars, 50 μm. Dotted lines indicate the regions measured in (d,e). (h,i) Scanning electron microscope images of IMs in NILipa1-2D (h) and NILIPA1 (i) at the IM3 stage in a. Scale bars, 100 μm.
