Figure 5: IPA1 dosage affects IPA characteristics.
From: A natural tandem array alleviates epigenetic repression of IPA1 and leads to superior yielding rice
(a) Morphology of plants carrying different IPA1 gDNA copy number. 1C, 2C, 3C, and 4C represent plants with 1, 2, 3 and 4 insertions of the transgenic construct, compared with the wild-type (WT) NIP. Scale bar, 15 cm. (b) Copy number detection of four lines by Southern blotting with WT as control. A probe from hygromycin phosphotransferase gene was used to detect the transgene. M, DNA markers. (c–e) IPA traits of plants with increasing IPA1 copy number, including tiller number (c), stem diameter (d) and panicle primary branch diameter (e). Values are means±s.d. (n=12). Different letters at top of each column indicate a significant difference at P<0.05 determined by Tukey’s HSD test. (f–h) Plots of relative IPA1 expression normalized to rice Actin with trait performance in different lines, including tiller number (f), stem diameter (g) and panicle primary branch number (h). Curves fitting the trait change are calculated by quadratic equation with R2 values.
