Figure 6: p140Cap impairs spontaneous metastasis.
(a,b) Representative gross observation (a,b), hematoxylin and eosin sections (c,d) and quantitative analysis in the experimental lung metastasis assay (a) and in the spontaneous lung metastasis assay (b). In a, 5 × 104 cells were injected into the tail vein of NSG mice. After 4 weeks, the lungs were explanted and analysed. Two independent experiments were performed using five mice per group. The histogram shows the percentage of metastatic lung tissue on total lung area. Statistical significance was evaluated with unpaired t-test (***P<0.001). Error bar: s.e.m. In b, 105 cells were injected in the right fat pads of NSG mice. Tumour volumes were measured every week; tumours were surgically removed when they reached a 10 mm diameter. After 5 weeks, mice were killed and lungs were explanted. The histogram shows the number of lung metastasis. Statistical significance was evaluated with unpaired t-test (*P<0.1). Error bar: s.e.m. Scale bar (a,b): 800 μm. (c) NeuT (a,c) and p140Cap expression (b,d) in spontaneous lung metastasis of mice injected with NeuT-TUBO cells (a,b) and mice injected with p140-TUBO cells (c,d). Scale bar, 400 μm. (d) High-magnification fields of rectangular areas in c, (panel d). Scale bar, 50 μm.
