Figure 1: H3K27ac ChIP-Seq profiling reveals upstream and downstream CD47 super-enhancers. | Nature Communications

Figure 1: H3K27ac ChIP-Seq profiling reveals upstream and downstream CD47 super-enhancers.

From: A CD47-associated super-enhancer links pro-inflammatory signalling to CD47 upregulation in breast cancer

Figure 1

(a) Heat map representing H3K27ac enrichment (grey to dark grey) across different cancer lines shows that T-ALL lines (RPMI-8402, Jurkat and MOLT3), the DLBCL line, LY4 and breast cancer lines (HCC1954 and MCF7) have SEs (red lines on top) associated with CD47. Green blocks: represent functional E5, E3.2 and E7 constituent enhancers from left to right respectively. (b) H3K27ac enrichment (y axis) shows that a downstream SE (red line on top) is associated with CD47 in an ER+ PR+ breast tumour sample (PDX1). Three other triple negative (TN=PR−, ER− and Her2−) breast tumour samples (PDX2-4) shown H3k27ac enrichment in the CD47 locus, but these genomic regions do not qualify as SEs. Right panel: H3K27ac ChIP-Seq binding profiles show typical enhancers at the KIT gene for size comparison between SEs and typical enhancers. Green blocks: represents the functional E5 constituent enhancer. Scale bars:10 kb.

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