Figure 5: ARPC1B-deficient platelets are microthrombocytes containing decreased numbers of dense granules. | Nature Communications

Figure 5: ARPC1B-deficient platelets are microthrombocytes containing decreased numbers of dense granules.

From: Loss of the Arp2/3 complex component ARPC1B causes platelet abnormalities and predisposes to inflammatory disease

Figure 5

(a) A comparison of platelet circumferential ring diameter distributions for all three ARPC1B mutant patients (P1, P2, P3) and a WASP-null patient (WAS) shows that all are significantly smaller (that is, microthrombocytes) compared to platelets from a normal donor (NC; P<0.0001, unpaired t-test; n=57 platelets, means and 95% confidence intervals shown by bars). No significant differences were observed among patients. (b) TEM of platelet whole mounts from an unaffected parent (left) and Patient 1 (right), showing dense granules detectable as dark spots (bar=500 nm; × 25,000). (c) Dense granule counts derived from whole-mount TEM imaging of platelets from Patient 1 (P1), Patient 2 (P2) and a WASP-null patient (WAS) were significantly lower than for cells from an unaffected relative (F1); Mann–Whitney test P<0.0008, n=50 platelets. Means for Patient 1 and WASP null were not significantly different (P=0.07), while the mean for Patient 2 was significantly higher than for Patient 1 (P<0.0001). Means and 95% confidence intervals shown by bars.

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