Figure 5: SCN infection of transgenic hairy roots of ExF50 expressing GmSNAP18 or AAT.

(a) SCN infection of RIL ExF50 transgenic hairy roots expressing Forrest GmSNAP18 or AAT. Vector-transformed ExF50 (SCN-susceptible) and ExF67 (SCN-resistant) hairy roots were used as controls. Each dot represents the number of cysts on a single hairy root line. The number of dots corresponds to the number of independent hairy root lines used. Mean values significantly different from the control were determined using an unpaired t-test (P<0.0004) and are denoted by different letters. Similar results were obtained from at least five independent experiments with Forrest GmSNAP18 and at least two independent experiments with AAT. Data from one representative experiment are shown. (b) SCN infection of RIL ExF50 transgenic hairy roots expressing GmSNAP18 of Forrest, Essex or PI 88788. Vector-transformed ExF50 (SCN-susceptible) hairy roots were used as a control. Each dot represents the number of cysts on a single hairy root line. The number of dots corresponds to the number of independent hairy root lines used. Mean values significantly different from the control were determined using an unpaired t-test (P<0.0015) and are denoted by different letters. Similar results were obtained from at least three independent experiments. Data from one representative experiment are shown.