Figure 7: Zbtb20 is required for carbohydrate-induced activation of glycolytic and lipogenic genes.
From: Regulation of hepatic lipogenesis by the zinc finger protein Zbtb20
3-4 month-old male control and LZB20KO mice were fasted for 24 h then or refed HCD for 18 h. n=5 per group. (a) Serum insulin levels were comparable between both genotypes. (b,c) mRNA expression levels of glycolytic and lipogenic genes (b) and transcriptional regulators (c). (d) Zbtb20 nuclear localization was increased after HCD feeding in control liver, and the activation and nuclear localization of ChREBP-α was impaired in LZB20KO liver, while SREBP1 was not affected. Lack of cytoplasmic protein contamination was confirmed in the nuclear extracts by probing tubulin (Supplementary Fig. 13). (e) Glucose-induced mRNA expression of glycolytic and lipogenic genes was decreased in LZB20KO hepatocytes in vitro. Primary hepatocytes from both genotypes were incubated under low glucose (5 mM) or high glucose (25 mM) conditions in the presence of 100 nM insulin for 6 h. n=5 per group. Error bars represent s.e.m. *P<0.05; **P<0.01 versus indicated groups (Student’s t-test).
