Figure 7: Depletion of FMNL2/3 in fibroblasts recapitulates migration defects.

(a) Box and whiskers plots summarizing quantification of random migration rates (μm min⁻¹) of double-knockout clones and wild-type NIH 3T3 cells as indicated. (b) Quantification of random migration speed (μm min⁻¹) of NIH 3T3 cells that were either migrating in regular medium or medium supplemented with different concentrations of polyvinylpyrrolidone (PVP) as indicated in the figure. Increase of PVP concentrations linearly correlates with decreased rates of random migration. As 1% PVP did not significantly affect migration of wild-type NIH 3T3 fibroblasts, this concentration was chosen for comparison with FMNL2/3 KO clones (red rectangle). (c) Box and whiskers plots summarizing quantification of random migration rates (μm min⁻¹) of double-knockout cell clones #9 and #46 as well as wild-type NIH 3T3 determined in regular medium versus medium of slightly increased viscosity (1% PVP), as indicated. Note the differential effect observed in medium of slightly increased viscosity (1% PVP) in KO clones versus wild-type cells (reduction of migration by 43% caused by 1% PVP in case of F2/F3 KO #9 and by 31% in case of F2/F3 KO #46). ***p<0.001 by Mann–Whitney rank sum test.