Figure 3: Lipid analysis in monocyte to macrophage differentiation.
From: Quantitative volumetric Raman imaging of three dimensional cell cultures
(a) qVRI identifies the main subcellular components of THP-1 cells (n=4 cells) and THP-1 differentiated macrophages (Mϕ) (n=4 cells) representative cells shown, and their corresponding (b) endmember Raman spectra from VCA (showing five components); from top to bottom cytoplasm (blue), nucleus (red), triacylglycerols (green), phospholipids (orange), cholesterol (magenta). (c) Bar chart of mean abundance values for each subcellular component showing significant differences between the two cells for TAG (two sample t-test, P<0.001), PLP (two sample t-test, P<0.001) and cholesterol (two sample t-test, P<0.001), ***P<0.001 (n/d, non-detectable). Error bars represent one standard deviation around the mean. Scale bar, 10 μm.
